Journal: Acta Medica Lituanica

Article Title: Immunohistochemical Evaluation of BARX1, DLX4, FOXE1, HOXB3, and MSX2 in Nonsyndromic Cleft Affected Tissue

doi: 10.15388/Amed.2022.29.2.13

Figure Lengend Snippet: HOXB3 immunopositive cells within the control group and cleft affected tissues. (3A) Control group with moderate HOXB3-containing epitheliocytes and few positive connective tissue cells (arrows), HOXB3 IMH, 200x. (3B) Unilateral cleft lip patient with a moderate number of HOXB3-containing epitheliocytes and the connective tissue cells, HOXB3 IMH, 200x. (3C) Bilateral cleft lip patient with moderate to numerous weakly stained HOXB3 epitheliocytes and a few positive connective tissue cells (arrows), HOXB3 IMH, 200x. (3D) Cleft palate patient with a moderate number of HOXB3-containing epitheliocytes and few to moderate HOXB3 positive connective tissue cells (arrows), HOXB3 IMH, 200x.

Article Snippet: The procedure of deparaffinization was conducted and the staining of slides was implemented by using biotin-streptavidin immunohistochemical method to ensure the detection cleft candidate gene proteins with antibodies for BARX1 (LS-C29810, 1:100, LifeSpan BioSciences, Inc., Seattle, WA, US), DLX4 (orb160775, 1:100, Biorbyt Ltd., Cambridge, UK), FOXE1 (ab5080, 1:500, Abcam, Cambridge, UK), HOXB3 (sc28606, 1:100, Santa Cruz Biotechnology, Dallas, TX, USA), MSX2 (ab22606, 1:100, Abcam, Cambridge, UK) Leica DC 300F digital camera was used to provide the visualization of slides.

Techniques: Control, Staining

Journal: Acta Medica Lituanica

Article Title: Immunohistochemical Evaluation of BARX1, DLX4, FOXE1, HOXB3, and MSX2 in Nonsyndromic Cleft Affected Tissue

doi: 10.15388/Amed.2022.29.2.13

Figure Lengend Snippet: Median values of semiquantitative evaluation for BARX1, DLX4, FOXE1, HOXB3, and MSX2 immunoreactivity within the control tissue group, unilateral cleft lip tissue group, bilateral cleft lip tissue group, and cleft palate tissue group.

Article Snippet: The procedure of deparaffinization was conducted and the staining of slides was implemented by using biotin-streptavidin immunohistochemical method to ensure the detection cleft candidate gene proteins with antibodies for BARX1 (LS-C29810, 1:100, LifeSpan BioSciences, Inc., Seattle, WA, US), DLX4 (orb160775, 1:100, Biorbyt Ltd., Cambridge, UK), FOXE1 (ab5080, 1:500, Abcam, Cambridge, UK), HOXB3 (sc28606, 1:100, Santa Cruz Biotechnology, Dallas, TX, USA), MSX2 (ab22606, 1:100, Abcam, Cambridge, UK) Leica DC 300F digital camera was used to provide the visualization of slides.

Techniques: Control

Journal: Acta Medica Lituanica

Article Title: Immunohistochemical Evaluation of BARX1, DLX4, FOXE1, HOXB3, and MSX2 in Nonsyndromic Cleft Affected Tissue

doi: 10.15388/Amed.2022.29.2.13

Figure Lengend Snippet: Statistically significant positive correlations in unilateral cleft lip tissue group (r s - Spearman’s rho value).

Article Snippet: The procedure of deparaffinization was conducted and the staining of slides was implemented by using biotin-streptavidin immunohistochemical method to ensure the detection cleft candidate gene proteins with antibodies for BARX1 (LS-C29810, 1:100, LifeSpan BioSciences, Inc., Seattle, WA, US), DLX4 (orb160775, 1:100, Biorbyt Ltd., Cambridge, UK), FOXE1 (ab5080, 1:500, Abcam, Cambridge, UK), HOXB3 (sc28606, 1:100, Santa Cruz Biotechnology, Dallas, TX, USA), MSX2 (ab22606, 1:100, Abcam, Cambridge, UK) Leica DC 300F digital camera was used to provide the visualization of slides.

Techniques:

Journal: Acta Medica Lituanica

Article Title: Immunohistochemical Evaluation of BARX1, DLX4, FOXE1, HOXB3, and MSX2 in Nonsyndromic Cleft Affected Tissue

doi: 10.15388/Amed.2022.29.2.13

Figure Lengend Snippet: Statistically significant positive correlations in bilateral cleft lip tissue group (r s - Spearman’s rho value).

Article Snippet: The procedure of deparaffinization was conducted and the staining of slides was implemented by using biotin-streptavidin immunohistochemical method to ensure the detection cleft candidate gene proteins with antibodies for BARX1 (LS-C29810, 1:100, LifeSpan BioSciences, Inc., Seattle, WA, US), DLX4 (orb160775, 1:100, Biorbyt Ltd., Cambridge, UK), FOXE1 (ab5080, 1:500, Abcam, Cambridge, UK), HOXB3 (sc28606, 1:100, Santa Cruz Biotechnology, Dallas, TX, USA), MSX2 (ab22606, 1:100, Abcam, Cambridge, UK) Leica DC 300F digital camera was used to provide the visualization of slides.

Techniques:

Journal: Acta Medica Lituanica

Article Title: Immunohistochemical Evaluation of BARX1, DLX4, FOXE1, HOXB3, and MSX2 in Nonsyndromic Cleft Affected Tissue

doi: 10.15388/Amed.2022.29.2.13

Figure Lengend Snippet: Statistically significant positive correlations in cleft palate tissue group (r s - Spearman’s rho value).

Article Snippet: The procedure of deparaffinization was conducted and the staining of slides was implemented by using biotin-streptavidin immunohistochemical method to ensure the detection cleft candidate gene proteins with antibodies for BARX1 (LS-C29810, 1:100, LifeSpan BioSciences, Inc., Seattle, WA, US), DLX4 (orb160775, 1:100, Biorbyt Ltd., Cambridge, UK), FOXE1 (ab5080, 1:500, Abcam, Cambridge, UK), HOXB3 (sc28606, 1:100, Santa Cruz Biotechnology, Dallas, TX, USA), MSX2 (ab22606, 1:100, Abcam, Cambridge, UK) Leica DC 300F digital camera was used to provide the visualization of slides.

Techniques: